Antiapoptotic and Antioxidant Properties of Orthosiphon stamineus Benth (Cat's Whiskers): Intervention in the Bcl-2-Mediated Apoptotic Pathway
Abdelwahab, SI; Mohan, S; Mohamed Elhassan, M; Al-Mekhlafi, N; Mariod, AA; Abdul, AB; Abdulla, MA; Alkharfy, KM
| HERO ID | 1455244 |
|---|---|
| In Press | No |
| Year | 2011 |
| Title | Antiapoptotic and Antioxidant Properties of Orthosiphon stamineus Benth (Cat's Whiskers): Intervention in the Bcl-2-Mediated Apoptotic Pathway |
| Authors | Abdelwahab, SI; Mohan, S; Mohamed Elhassan, M; Al-Mekhlafi, N; Mariod, AA; Abdul, AB; Abdulla, MA; Alkharfy, KM |
| Journal | Evidence-Based Complementary and Alternative Medicine |
| Volume | 2011 |
| Page Numbers | 156765 |
| Abstract | Antiapoptotic and antioxidant activities of aqueous-methanolic extract (CAME) of Orthosiphonstamineus Benth(OS), and its hexane (HF), chloroform (CF), n-butanol (NBF), ethyl acetate (EAF) and water (WF) fractions were investigated. Antioxidant properties were evaluated using the assays of Folin-Ciocalteu, aluminiumtrichloride, β-carotene bleaching and DPPH. The role of OS against hydrogen peroxide induced apoptosis on MDA-M231 epithelial cells was examined using MTT assay, phase contrast microscope, colorimetric assay of caspase-3, western blot and quantitative real-time PCR. Results showed that EAF showed the highest total phenolic content followed by CAME, NBF, WF, CF and HF, respectively. Flavonoid content was in the order of the CF > EAF > HF > CAME > NBF > WF. The IC(50) values on DPPH assay for different extract/fractions were 126.2 ± 23, 31.25 ± 1.2, 15.25 ± 2.3, 13.56 ± 1.9, 23.0 ± 3.2, and 16.66 ± 1.5 μg/ml for HF, CF, EAF, NBF, WF and CAME, respectively. OSreduced the oxidation of β-carotene by hydroperoxides. Cell death was dose-dependently inhibited by pretreatment with OS. Caspase-3 and distinct morphological features suggest the anti-apoptotic activities of OS. This plant not only increased the expression of Bcl-2, but also decreased Bax expression, and ultimately reduced H(2)O(2)-induced apoptosis. The current results showed that phenolics may provide health and nutritional benefits. |
| Doi | 10.1155/2011/156765 |
| Pmid | 21234328 |
| Wosid | WOS:000293302200001 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Journal: Evidence-based complementary and alternative medicine : eCAM ISSN: 1741-4288Scopus URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-79952225939&doi=10.1155%2f2011%2f156765&partnerID=40&md5=b863b9e2afd2f63afd0478da55e55ee8 |
| Is Public | Yes |
| Language Text | English |