Inhibitory Effects of Ginsenoside Rb-1, Rg(3), and Panax ginseng Head Butanol Fraction on Inflammatory Mediators from LPS-Stimulated RAW 264.7 Cells
Lee, JeH; Jeong, C
| HERO ID | 1456022 |
|---|---|
| In Press | No |
| Year | 2008 |
| Title | Inhibitory Effects of Ginsenoside Rb-1, Rg(3), and Panax ginseng Head Butanol Fraction on Inflammatory Mediators from LPS-Stimulated RAW 264.7 Cells |
| Authors | Lee, JeH; Jeong, C |
| Volume | 16 |
| Issue | 3 |
| Page Numbers | 277-285 |
| Abstract | Panax ginseng C.A. Mayer (Araliaceae, R ginseng) has been used for the enhancement of vascular and immune functions in Korea and Japan for a long time. Ginsenoside Rb-1 and Rg(3) isolated from P ginseng head-part butanolic extract (PGHB) were investigated for anti-inflammatory activity. Ginsenosides and PGHB did not affect the cell viability within 0 - 100 mu g/ml concentration to RAW 264.7 murine macrophage cells. Ginsenosides and PGHB inhibited partly lipopolysaccharide (LPS)-induced nitrite production in a dose-dependent manner. The ginsenosides and PGHB showed partially chemical nitric oxide (NO) quenching (maximum 40%) in the cell-free system. Also, ginsenoside Rb, and Rg(3) inhibited markedly approximately 74 and 54% of inducible nitric oxide synthase (iNOS) mRNA transcription from LPS-induced RAW 264.7 cells. Taken together, the inhibitory effect of ginsenosides and PGHB on NO production did not occur as a result of cell viability, but was caused by both the chemical NO quenching and the regulation of iNOS. Additionally, the ginsenoside Rb, and PGHB inhibited prostaglandin E-2 (PGE (2)) synthesis in a concentration-dependent manner, showed approximately 70-98% inhibition at 100 mu g/ml concentration. And the treatment with ginsenosides and PGHB attenuated partially LPS- upregulated cyclooxygenase-2 (COX-2) gene transcription. Ginsenoside Rg(3) suppressed LIPS- stimulated intedeukin-6 (IL-6) level to the basal in RAW 264.7 cells. From these results, ginsenoside Rb-1, Rg(3), and PGHB may be useful for the relief and retardation of immunological inflammatory responses and its action may occur through the reduction of inflammatory mediators, including NO, PGE2, and IL-6 production. |
| Doi | 10.4062/biomolther.2008.16.3.277 |
| Wosid | WOS:000260150600018 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:000260150600018 Journal:BIOMOLECULES & THERAPEUTICS 1976-9148 |
| Is Public | Yes |
| Keyword | Ginsenoside Rb-1; Ginsenoside Rg(3); Prostaglandin E-2; Nitric oxide; iNOS; COX-2; IL-6 |