On-line concentration and determination of tobacco-specific N-nitrosamines by cation-selective exhaustive injection-sweeping-micellar electrokinetic chromatography
Yang, Y; Nie, H; Li, C; Bai, Yu; Li, N; Liao, Jie; Liu, H
| HERO ID | 1461562 |
|---|---|
| In Press | No |
| Year | 2010 |
| Title | On-line concentration and determination of tobacco-specific N-nitrosamines by cation-selective exhaustive injection-sweeping-micellar electrokinetic chromatography |
| Authors | Yang, Y; Nie, H; Li, C; Bai, Yu; Li, N; Liao, Jie; Liu, H |
| Journal | Talanta |
| Volume | 82 |
| Issue | 5 |
| Page Numbers | 1797-1801 |
| Abstract | In this paper, a micellar electrokinetic chromatography (MEKC) method combined with cation-selective exhaustive injection (CSEI) and sweeping was developed to separate and concentrate four tobacco-specific N-nitrosamines (TSNAs) including N'-nitrosoanabasine (NAB), 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) and 4-(methylnitrosamino)-4-(3-pyridyl)-1-butanol (iso-NNAL). Experimental parameters affecting separation efficiency and enhancement factors were investigated in detail. Under the optimum MEKC condition, NAB, NNK, NNAL and iso-NNAL were baseline separated with high separation efficiencies and good peak shapes. Furthermore, with the preconcentration by CSEI-sweeping-MEKC, the sensitivity enhancement factors for NAB, NNK, NNAL and iso-NNAL in terms of peak areas ranged from 6.0×10(3) to 1.5×10(4), and the detection limits (LOD, S/N=3) of four TSNAs were in the range of 0.004-0.016μg/mL. In addition, this method had fairly good repeatability, and the RSDs of retention time and peak area were less than 1% and 5%, respectively. Finally, this method showed promising capabilities in the application of detecting and analyzing TSNAs in human urine samples. |
| Doi | 10.1016/j.talanta.2010.07.075 |
| Pmid | 20875579 |
| Wosid | WOS:000283903300025 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:000283903300025 |
| Is Public | Yes |
| Language Text | English |
| Keyword | Cation selective exhaustive injection; Micellar electrokinetic chromatography; On-line concentration; Sweeping; Tobacco-specific N-nitrosamine |