Gas chromatography/mass spectrometry screening of serum metabolomic biomarkers in hepatitis B virus infected cirrhosis patients
Xue, R; Dong, L; Wu, H; Liu, T; Wang, J; Shen, X
| HERO ID | 659165 |
|---|---|
| In Press | No |
| Year | 2009 |
| Title | Gas chromatography/mass spectrometry screening of serum metabolomic biomarkers in hepatitis B virus infected cirrhosis patients |
| Authors | Xue, R; Dong, L; Wu, H; Liu, T; Wang, J; Shen, X |
| Journal | Clinical Chemistry and Laboratory Medicine |
| Volume | 47 |
| Issue | 3 |
| Page Numbers | 305-310 |
| Abstract | Background: Metabolomic studies have been applied to disease biomarkers selection. With the metabolomic technique, gas chromatography/mass spectrometry (GC/MS), human serum metabolites can be detected and identified. The purpose of this study was to investigate the serum metabolic profile of hepatitis B virus (HBV) infected cirrhosis patients and to detect disease biomarkers. Methods: HBV infected non-cirrhosis male subjects (n=20) and HBV infected cirrhosis male patients (n=20) participated in this experiment. Serum metabolome was detected through chemical derivatization followed by GC/MS. The high-flux metabolomic data were analyzed by stepwise discriminant analysis. Results: Out of the 41 metabolites detected in serum, we selected metabolites, including acetic acid, sorbitol, D-lactic acid, hexanoic acid, 1-naphthalenamine, butanoic acid, phosphoric acid, D-glucitol, and glucose, which in combination with each other could segregate the two groups. The error count was 0% for the non-cirrhosis group and 25% for the cirrhosis group. Conclusions: This technique can be used to select biomarkers for hepatic cirrhosis. Clin Chem Lab Med 2009;47. |
| Doi | 10.1515/CCLM.2009.08 |
| Pmid | 19676142 |
| Wosid | WOS:000265031000010 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:000265031000010 |
| Is Public | Yes |
| Language Text | English |
| Keyword | biomarker; cirrhosis; gas chromatography/mass spectrometry; hepatitis B virus (HBV); metabolomic |
| Is Qa | No |