[Screening of Clostridium strains through ribosome engineering for improved butanol production]
Chen, L; Shang, G; Yuan, W; Wu, Y; Bai, F
| HERO ID | 1454352 |
|---|---|
| In Press | No |
| Year | 2012 |
| Title | [Screening of Clostridium strains through ribosome engineering for improved butanol production] |
| Authors | Chen, L; Shang, G; Yuan, W; Wu, Y; Bai, F |
| Journal | Shengwu Gongcheng Xuebao / Chinese Journal of Biotechnology |
| Volume | 28 |
| Issue | 9 |
| Page Numbers | 1048-1058 |
| Abstract | We used ribosome engineering technology, with which antibiotic-resistant strains are resulted from mutations on microbial ribosome, to screen a high butanol-producing Clostridium strain. A novel mutant strain S3 with high butanol production and tolerance was obtained from the original Clostridium acetobutylicum L7 with the presence of mutagen of streptomycin. Butanol of 12.48 g/L and ethanol of 1.70 g/L were achieved in S3, 11.2% and 50%, respectively higher than the parent strain. The conversion rate of glucose to butanol increased from 0.19 to 0.22, and fermentation time was 9 h shorter. This caused an increase in butanol productivity by 30.5%, reaching 0.24 g/(Lh). The mutant butanol tolerance was increased from 12 g/L to 14 g/L, the viscosity of fermentation broth was dramatically decreased to 4 mPa/s, 60% lower than the parent strain. In addition, the genetic stability of mutant strain S3 was also favorable. These results demonstrate that ribosome engineering technology may be a promising process for developing high butanol-producing strains. |
| Pmid | 23289307 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | chi |