[Effect of sodium butyrate on apoptosis and stromal interaction molecule and Orai1 activity in human colon cancer HCT-116 cells in vitro]

Sun, S; Li, W; Chen, S; Zhang, H; Yu, S; Zhang, M; Zou, F

HERO ID

1455006

Reference Type

Journal Article

Year

2012

Language

chi

PMID

22381755

HERO ID 1455006
In Press No
Year 2012
Title [Effect of sodium butyrate on apoptosis and stromal interaction molecule and Orai1 activity in human colon cancer HCT-116 cells in vitro]
Authors Sun, S; Li, W; Chen, S; Zhang, H; Yu, S; Zhang, M; Zou, F
Journal Nan Fang Yi Ke Da Xue Xue Bao / Journal of Southern Medical University
Volume 32
Issue 2
Page Numbers 189-192
Abstract <strong>OBJECTIVE: </strong>To investigate the mechanism underlying sodium butyrate (NaB)-induced apoptosis of a human colon cancer cell line HCT-116.<br /><br /><strong>METHODS: </strong>The apoptosis of HCT-116 cells induced by NaB was confirmed by hoechst33342 staining and AnnexinV+ PI assay. The changes in the intracellular localization of stromal interaction molecule (STIM1) and Orai1 following NaB treatment were detected by immunofluorescence technique. Western blotting was used to investigate the protein expression levels of STIM1 and Orai1.<br /><br /><strong>RESULTS: </strong>NaB induced apoptosis and caused translocation and colocalization of STIM1 and Orai1 in HCT-116 cells.<br /><br /><strong>CONCLUSION: </strong>The apoptosis of human colon cancer cells induced by NaB is correlated to the redistribution of STIM1 and Orai1.
Pmid 22381755
Is Certified Translation No
Dupe Override No
Is Public Yes
Language Text chi