[Determination of rat serum emodin level by microemulsion liquid chromatography with direct sample loading]
Shi, Y; Wang, BJ; Lin, XK; Huang, SX; Lin, KQ; Zhang, SY
HERO ID
1455261
Reference Type
Journal Article
Year
2010
Language
chi
PMID
| HERO ID | 1455261 |
|---|---|
| In Press | No |
| Year | 2010 |
| Title | [Determination of rat serum emodin level by microemulsion liquid chromatography with direct sample loading] |
| Authors | Shi, Y; Wang, BJ; Lin, XK; Huang, SX; Lin, KQ; Zhang, SY |
| Journal | Nan Fang Yi Ke Da Xue Xue Bao / Journal of Southern Medical University |
| Volume | 30 |
| Issue | 12 |
| Page Numbers | 2759-2761 |
| Abstract | <strong>OBJECTIVE: </strong>To establish a microemulsion liquid chromatography system with direct sample loading for determining the serum level of emodin in rats.<br /><br /><strong>METHODS: </strong>The separation was performed on C₁₈ column (Hypersil BDS, 5 µm,150 mm×4.6 mm) with the microemulsion mobile phase consisting of 3.3% (w/V) SDS, 6.6% (V/V) n-butyl alcohol, and 1.0% (V/V) octane and water. The flow rate was 1.0 ml/min and the detection wavelength was 254 nm.<br /><br /><strong>RESULTS: </strong>The linear range of emodin detection was 0.333-5.32 µg/ml. The average recovery was 99.65% with a RSD of 3.60%. The limit of quantification was 0.1386 µg/mL.<br /><br /><strong>CONCLUSION: </strong>Microemulsion liquid chromatography system with direct sample loading allows simple, accurate and rapid determination of emodin in rat serum. |
| Pmid | 21177199 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | chi |